Isolation, Identification and Antibiogram of Pasteurella Multocida Isolates of Avian Origin
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چکیده
Isolation of Pasteurella multocida was attempted from the heart blood, spleen, liver and lung collected from turkeys (4 No,s) and chicken (4 No,s) suspected to have died of avian pasteurellosis. A total of eight P. multocida isolates were isolated and identified on the basis of biochemical characteristics, pathogenicity studies in mice and PM-PCR. The In vitro antibiotic sensitivity test of the organisms was conducted which revealed that all the isolates showed sensitivity to a wide range of antibiotics namely Enrofloxacin (100.00 per cent), Ciprofloxacin (100.00 per cent), Oflaxacin (100.00 per cent), Gentamicin (100.00 per cent), Amikacin (100.00 per cent), Ampicillin (100.00 per cent) and Penicillin (100.00 per cent). Both turkey isolates and chicken isolates showed sensitivity to Oxytetracycline (50.00 per cent) and Doxycycline (50.00 per cent) whereas all the turkeys and chicken isolates showed resistance to Chloramphenicol, Poymixin B, Trimethoprim, Erythromycin and Triple sulpha. Hence this study recommended to perform in vitro antibiotic sensitivity test prior to treatment in cases of fowl cholera. If not feasible, Fluoroquinolones, Gentamicin, Amikacin, Ampicillin and Penicillin must be given preferential consideration over other antibiotics particularly Chloramphenicol, Poymixin B, Trimethoprim, Erythromycin and Triple sulpha to contain the outbreak of Fowl cholera.
منابع مشابه
Prevalence of adhesion Virulence factor genes, antibiogram, and pathogenicity of avian Pasteurella multocida isolate from Iran
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